Other reagents utilized and their sources were the following: anti-Flag M2 affinity gel (Sigma, A2220), DAPT (Sigma, D5942), LY-411575 (Sigma, SML0506), NF-B inhibitor BAY 11C7082 (Beyotime, S1523), Wnt inhibitor Salinomycin (Sigma, S4526), and TNF (Peprotech, 315-01A)
Other reagents utilized and their sources were the following: anti-Flag M2 affinity gel (Sigma, A2220), DAPT (Sigma, D5942), LY-411575 (Sigma, SML0506), NF-B inhibitor BAY 11C7082 (Beyotime, S1523), Wnt inhibitor Salinomycin (Sigma, S4526), and TNF (Peprotech, 315-01A). Quantitative real-time PCR (qPCR) Cells were harvested and lysed using the TRIzol reagent (Existence Systems). in iSLK.RGB cells by…