After ligand treatment, plates were washed in PBS, and staying cell surface biotinylated receptors were stripped in 50 mM glutathione/0
After ligand treatment, plates were washed in PBS, and staying cell surface biotinylated receptors were stripped in 50 mM glutathione/0.3 M NaCl/75 mM NaOH/1% FBS at 4C for 30 min. low dosages of D2R agonists (8). As a result, we hypothesized that D2Rs could be targeted for degradation in the same PSI-7409 way as reported…