Plasmid-encoded HIV-1 protease was expressed as inclusion bodies in 1458 (13, 15, 18)
Plasmid-encoded HIV-1 protease was expressed as inclusion bodies in 1458 (13, 15, 18). subset of these amino acid polymorphisms (M36I/R41K/H69K/L89M), which are found in subtype C and other HIV subtypes, also was studied. Both proteases were found to have similar catalytic constants, site-directed mutagenesis kit (Stratagene), and mutations were confirmed by DNA sequencing. Proteases were…