The precipitate was recovered by centrifugation at 13,000?rpm for 10 minutes, washed twice with 75% ethanol then resuspended in 12
The precipitate was recovered by centrifugation at 13,000?rpm for 10 minutes, washed twice with 75% ethanol then resuspended in 12.5?L RIPA buffer for electrophoresis about 10% SDS gels and transferred to PVDF membrane. chondrocytes with the calcium ionophore, ionomycin. Furthermore, we display that in addition to the lysosomal exocytosis markers, cathepsin D and -hexosaminidase, ionomycin…