Positive clones were recognized from the PCR, and the final plasmid was confirmed by DNA sequencing
Positive clones were recognized from the PCR, and the final plasmid was confirmed by DNA sequencing. We injected Cas-9-sgRNA, homologous recombination template, and the selection marker pRF4 (place, and primers P2, P3, and P4 to display their progeny for homozygotes (S2 Table). DIC, differential interference contrast.(TIF) pbio.2005069.s001.tif (1.5M) GUID:?A69CC937-2DFC-4788-880D-49B337D0076F S2 Fig: Labile zinc colocalizes with…